Showing 4 results for Mazandarani
Mohammad Mazandarani, Mohammad Sudagar, Somayeh Namroodi,
Volume 5, Issue 1 (ُSummer 2015)
Abstract
In the present study histological effects of copper sulphate on, 180 common carp, Cyprinus carpio, fingerlings were studied. In this regard, fish of treatment groups were exposed to 2, 4, 6, 8 and 10 ppm of copper sulphate during 4 days. Behaviours and clinical signs of fish were recorded during the experiment, and samples were done from kidney, liver and gill of moribund and fresh dead fish, and specimens were studied after section and hematoxylin_ Eosin staining for histopathological signs. In this study LC50 value for 48 and 96 hours exposure were calculated as 6.3 and 4.9 mg/l, respectively for common carp fingerlings. Mild to severe histopathological signs were observed in kidney, liver and gill of all treatment fish specimens. Pathologic severity signs in sampled tissues were increased by increasing in concentration and exposure times of copper sulphate. According to the results, it is not recommended to use copper sulphate in fingerling common carp farms in dosage higher than 2 mg/l.
Mohammad Mazandarani, Ali Taheri Mirghaeed,
Volume 5, Issue 4 (Spring 2016)
Abstract
In the present study, we evaluated the pathogenicity of Yersinia ruckeri on Persian sturgeon (Acipenser persicus) fingerlings. We divided the sturgeon into one treatment group, one positive control group and one negative control group (with three replicates for each group). The treatment group juvenile Persian sturgeon received 0.1 ml of bacterial suspension (2.8 × 106 cells/fish) via an intraperitoneal injection. Positive control fish received 0.1 ml intraperitoneal injection of sterile normal saline. The negative control group received no injection. According to the results, first mortality was observed 48 h post-challenge in the treatment group. Mortality rates in the treatment group post-challenge were 6.7% (48 h), 26.6% (72 h), 60% (96 h), and 70% (7 days). Haemorrhage in terminal part of intestine and accumulation of bloody fluid in the intestinal lumen were observed in the majority of sick fish. Intestinal histopathology was characterized by spread necrosis in the epithelium of the villi and lymphocytic infiltration to the connective tissues. In the haematological study, we observed significant differences in RBC, WBC, MCH, MCHC, hemoglobin, and hematocrit counts between fish from the treatment and control groups.
Fatemeh Khodadadi Arpanahi, Mohammad Mazandarani, Valiollah Jafari, Korosh Amini,
Volume 8, Issue 3 (Winter 2019)
Abstract
The present study investigated the susceptibility of Caspian roach, Rutilus caspicus to experimental exposure of Yersinia ruckeri. In this regard, 144 Caspian roach fingerlings with average weight of 9.3 ± 2.1 were assigned to 12 aquaria (12 fish per aquarium). Fish were acclimated for 4 weeks before challenge. Five experimental treatments and one control group (each with two replicates) were considered. After preparation of bacterial suspension, fish were challenged via intra peritoneal injection with different dosages of bacteria including 5.4×108, 5.4×107, 5.4 ×106, 5.4 ×105 and 5.4 ×104 cells/fish. Fish in control group received sterile normal saline. After 21 days, the median lethal concentration (LC50) of this bacterium calculated as 4.4×107. The most gill histopathology signs in sick fish included epithelial lifting and inflammatory cell infiltration in secondary lamella and hyperplasia in base of secondary lamella. Kidney histopathology was characterized by congestion in vessels and interstitial tissue, focal necrosis and inflammatory cell infiltration. In liver tissue, vacuolization of hepatocytes was observed in most specimens and focal hepatocyte necrosis was recorded in some individuals.
- Bita Kalvani Neital, Mohammad Sudagar, Mohammad Mazandarani, Ahmad Nouri, Mercedes Gonzalez-Wanguemert,
Volume 9, Issue 2 (Autumn 2019)
Abstract
Female sea cucumbers maintain their oocytes in meiosis prophase I, and their maturation only occurs just before spawning. A new methodology for inducing in vitro oocyte maturation in sea cucumbers is to expose them to their nerve extract which contains gonad stimulating substance (GSS) that leads to final oocyte maturation. Since the extract should be obtained only in the reproduction season of the year, it is important to cryopreserve this extract. The objective of this work was to assess the effects of short freezing on oocyte maturation induction of radial nerve extract of Holothuria arguinensis. The sea cucumbers were sampled during summer from Ria Formosa (Faro, S Portugal), radial nerves dissected and their extract obtained. A fraction of the extract was used immediately (fresh) and the other fraction was used after freezing at -20˚C. Then the oocytes were treated with fresh and frozen nerve extracts at different concentrations including 0%, 50%, and 100% for 90 min. The appearance of germinal vesicle breakdown (GVBD) was considered as an oocyte final maturation indicator. The results did not show any significant differences between the two groups (fresh and frozen extract). Therefore, freezing the nerve extract in the reproduction season might have no adverse effects on the function of GSS on inducing oocyte maturation, and this extract can be used for a long time.
