Showing 2 results for Alkaline Phosphatase
Zahra Roosta, Abdolmajid Hajimoradloo, Seyed Hossein Hoseinifar, Farzaneh Vakili,
Volume 3, Issue 2 (10-2013)
Abstract
Fish epidermal mucus and its components provide the first defense line against pathogens. This study was conducted to evaluate the effects of different levels of Lactobacillus acidophilus on antibacterial activity, MIC and some immune parameters of mucus in Puntius tetrazona. Six hundred and thirty fish (0.5 ± 0.03 g) were randomly distributed in 9 aquarium for 3 treatments with 3 replicates (n=70). Fish were fed for 90 days with probiotic supplemented diets (0, 1.5×108and 3×108). At the end of the experiment antibacterial activity against Gram-positive and Gram-negative pathogen Streptococcus faecium and Escherichia coli, respectively, MIC, alkaline phosphatase activity and mucus protein levels were measured. A disk diffusion and microdilution method was used for antibacterial determination. Growth inhibition zone diameters of mucus significantly increased against both bacteria along with increase of probiotic concentration. The serial dilution of 12.5, 25, 50, 100, 200 µl/ml were considered. Alkaline phosphatase activity and mucus protein levels showed a significant differences between probiotic fed fish and control group (P< 0.05).The results showed that the mucus of Puntius tetrazona have an antibacterial activity against the two bacteria strains and fish fed Lactobacillus acidophilus supplemented diet showed elevated mucus antibacterial activity compared fish fed control diet.
Miss Samira Yousefi, Seyed Hossein Hoseinifar, Dr. Abdolmajid Hajimoradloo, Hamed Kolangi Miandare,
Volume 7, Issue 2 (10-2017)
Abstract
The aim of the present study was to evaluate the effects of different levels of prebiotic on growth performance and non-specific immune parameters of zebra fish. Four hundred and twenty fish (mean weight 45 ± 0.1 mg) were supplied, randomly stocked in aquaria assigned to four treatments repeated in triplicates and fed with experimental diets contain 0, 0.5, 1 and 2% prebiotic for 8 weeks. At the end of feeding trial, growth performance and feed utilization parameters (final weight, specific growth rate and feed conversion ratio) as well as non-specific immune parameters (total immunoglobulin, total protein and alkaline phosphatase activity) were measured. The results revealed that dietary administration of prebiotic had no significant effect on growth performance (P>0.05). Also, the study of non-specific immune parameters revealed that inclusion of 0.5 and 2% prebiotic in diet remarkably increased total Ig (P<0.05). Feeding on 2% prebiotic significantly increased ALP activity (P<0.05). Regardless of inclusion level, prebiotic administration in zebrafish diet elevated total protein levels (P<0.05). According to these results it seems that inclusion of higher prebiotic levels (2%) increase non-specific immune parameters in zebra fish.
