Showing 2 results for Reproductive Toxicity
Nasrin Hassanzadeh,
Volume 6, Issue 3 (4-2016)
Abstract
Paraben compounds are used widely as preservatives to inhibit microbial growth and extend used in pharmaceuticals, hygienic, cosmetics, care products. They pose the potential for reproductive toxicity for users. This paper provides an overview of the applications of histopathological biomarkers in reproductive toxicity in zebrafish caused by methylparaben. The present study investigated the changes in ovarian histopathology and Gonad Somatic Index (GSI) in zebrafish (Danio rerio) adult female after exposure to 0.001, 0.01, 1 and 10 mg/L methylparaben for 3 weeks in semi-static condition. Histological preparations were stained with Hematoxylin & Eosin. The results showed that Treatment at 0.001 to 10 mg/L of methylparaben had no significant effect on the survival, and condition factor. But in all treatments, GSI decreased and ovarian complications include wrinkled ooplasm, impaired yolk production, granulomatous inflammation and proteinaceous fluid were observed in the interstitial space of treated zebrafish ovaries. Also, atresia of vitellogenic oocytes was determined. These data show that an increase in atretic follicles has negative effects on zebrafish fertility and continuous exposure to subacute sublethal concentrations of methylparaben increase estrogenic and irreversible effects on the reproductive in female zebrafish.
Nasrin Hassanzadeh,
Volume 7, Issue 2 (10-2017)
Abstract
This study aimed to investigatethe changes in Gonad Somatic Index (GSI), vitellogenin induction and ovarian histopathology in zebrafish (Danio rerio) adult female, as an ecological model in toxicology, after exposure to 0.002, 0.02, 0.2, 2 and 20 µg/L cadmium chloride for 21 days under sub-lethal condition. The results showed that 0.002 to 20 µg/L cadmium chloride had no significant effect on the survival, But GSI and vitellogenin concentrations in whole body homogenate (WBH) decreased in a dose- dependent manner in all treatments. Gonad histology showed increased proportion of atretic follicles, reduced proportion of vitellogenic oocytes, haemorrhage, hyperemia, membrane folding in some oocytes, hypertrophy of granulosa cells, proteinaceous fluid and decreased yolk formation in vitellogenic oocytes. These changes indicate a disturbed reproductive output. The results of this study showed that an increase in atretic follicles and decreased vitellogenin have negative effects on zebrafish fertility. Moreover,continuous exposure to subacute sublethal concentrations of cadmium chloride had anti-estrogenic and irreversible effects on zebrafish reproduction.
