Showing 4 results for Hajimoradloo
Zahra Roosta, Abdolmajid Hajimoradloo, Seyed Hossein Hoseinifar, Farzaneh Vakili,
Volume 3, Issue 2 (Autumn 2013)
Abstract
Fish epidermal mucus and its components provide the first defense line against pathogens. This study was conducted to evaluate the effects of different levels of Lactobacillus acidophilus on antibacterial activity, MIC and some immune parameters of mucus in Puntius tetrazona. Six hundred and thirty fish (0.5 ± 0.03 g) were randomly distributed in 9 aquarium for 3 treatments with 3 replicates (n=70). Fish were fed for 90 days with probiotic supplemented diets (0, 1.5×108and 3×108). At the end of the experiment antibacterial activity against Gram-positive and Gram-negative pathogen Streptococcus faecium and Escherichia coli, respectively, MIC, alkaline phosphatase activity and mucus protein levels were measured. A disk diffusion and microdilution method was used for antibacterial determination. Growth inhibition zone diameters of mucus significantly increased against both bacteria along with increase of probiotic concentration. The serial dilution of 12.5, 25, 50, 100, 200 µl/ml were considered. Alkaline phosphatase activity and mucus protein levels showed a significant differences between probiotic fed fish and control group (P< 0.05).The results showed that the mucus of Puntius tetrazona have an antibacterial activity against the two bacteria strains and fish fed Lactobacillus acidophilus supplemented diet showed elevated mucus antibacterial activity compared fish fed control diet.
Zahra Roohi, Mohammad Reza Imanpoor, Abdolmajid Hajimoradloo, Mahbubeh Salmanian Ghahderijani,
Volume 6, Issue 1 (Summer 2016)
Abstract
In this study, we investigated the effects of herbal supplements of Carum carvi and Trigonella foenum graecum on soluble protein and antibacterial activity of mucus in common carp (Cyprinus carpio) fingerlings. Fish with a mean weight of 2.457±0.057 g were divided randomly into 7 treatments (one control group and the experimental groups treated with diets that contained different percents of caraway and fenugreek (0.5, 1 and 1.5%) with 3 replicates and a density of 15 fish per replicate. After 8 weeks feeding, the epidermal mucus protein level and antimicrobial activity against two gram-negative bacteria (Escherichia coli and Seratia marcescens) were measured. Results showed that protein levels of mucus significantly increased in fish fed diets that contained 1% herbal supplements compared to the control and other treatments (P>0.05). In addition, we observed significant differences in antibacterial activity of mucus in fish fed with caraway and fenugreek compared with the control group (p<0.05). The highest antibacterial activity of mucus was observed in fish fed 1% caraway and 1% fenugreek. These results revealed that caraway and fenugreek beneficially affected the protein level and antibacterial activity of skin mucus of common carp fingerling.
Miss Samira Yousefi, Seyed Hossein Hoseinifar, Dr. Abdolmajid Hajimoradloo, Hamed Kolangi Miandare,
Volume 7, Issue 2 (Autumn 2017)
Abstract
The aim of the present study was to evaluate the effects of different levels of prebiotic on growth performance and non-specific immune parameters of zebra fish. Four hundred and twenty fish (mean weight 45 ± 0.1 mg) were supplied, randomly stocked in aquaria assigned to four treatments repeated in triplicates and fed with experimental diets contain 0, 0.5, 1 and 2% prebiotic for 8 weeks. At the end of feeding trial, growth performance and feed utilization parameters (final weight, specific growth rate and feed conversion ratio) as well as non-specific immune parameters (total immunoglobulin, total protein and alkaline phosphatase activity) were measured. The results revealed that dietary administration of prebiotic had no significant effect on growth performance (P>0.05). Also, the study of non-specific immune parameters revealed that inclusion of 0.5 and 2% prebiotic in diet remarkably increased total Ig (P<0.05). Feeding on 2% prebiotic significantly increased ALP activity (P<0.05). Regardless of inclusion level, prebiotic administration in zebrafish diet elevated total protein levels (P<0.05). According to these results it seems that inclusion of higher prebiotic levels (2%) increase non-specific immune parameters in zebra fish.
Hamed Paknejad, Abdolmajid Hajimoradloo, Soheila Rastgari, Marjan Hosseini, Hamed Azadi,
Volume 8, Issue 4 (ُُSpring 2019)
Abstract
In recent years, the administration of medicinal herbs as growth and innate immune stimulator has been increasing in fish. In this study, the effect of peppermint powder on GH and IGF gene expression and hematology was studied In Caspian roach (Rutilus caspicus). Fish fed diets containing 0 (control), 2, 3 and 4 g/kg for 8 weeks. At the end of the experiment, blood sampling was done and liver and brain tissues were dissected in a sterile condition. Results indicated that peppermint powder upregulated GH and IGF expression and there were significant differences among treatmentss and control group (P˂0.05). The results of hematology assay showed that there were remarkable differences in Hct, Hb, RBC and WBC in fish fed diet containing power peppermint and control (P˂0.05). In conclusion, the administration of powder peppermint increased the growth and innate immune parameters of Caspian roach.