The aim of this study was to examine the accumulation of lead, nickel, and cadmium heavy metals in the muscle tissue of Peronia peronii in the waters of Bandar Lange and Qeshm in April. Thirty samples were separately collected from each station and subjected to drying and acid digestion before measurement using a flame atomic absorption device (GBC model F savant). The results revealed varying levels of metal accumulation, with the highest and lowest concentrations recorded as 0.112 and 0.024 micrograms per gram for nickel and cadmium, respectively, in Langeh and Qeshm ports. Additionally, a significant relationship between the concentration of nickel, lead, and cadmium and the average total weight of P. peronii was observed, indicating a positive correlation with the total weight (p<0.05). However, no significant relationship was found between the accumulation of these metals and the average total length of P. peronii in Bandar Lange and Qeshm Island, indicating a lack of correlation with the total length of the flounder seaweed (p>0.05).