Identification of “alpha-conotoxin-like” Peptide in Conus pennaceus Born, 1778, Venom

Dehghani, Hadi; Fazeli, Mehdi; Rajaeian, Hamid; Hosseinzadeh, Saeid; Ashrafi, Mahboobeh

Volume 8, Issue 2 (Autumn 2018) J. Aqua. Eco 2018, 8(2): 105-114 | Back to browse issues page

‎ 20.1001.1.23222751.1397.8.2.10.4

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Dehghani H, Fazeli M, Rajaeian H, Hosseinzadeh S, Ashrafi M. Identification of “alpha-conotoxin-like” Peptide in Conus pennaceus Born, 1778, Venom. J. Aqua. Eco 2018; 8 (2) :105-114
URL: http://jae.hormozgan.ac.ir/article-1-719-en.html

Identification of “alpha-conotoxin-like” Peptide in Conus pennaceus Born, 1778, Venom

Hadi Dehghani

, Mehdi Fazeli ^*

, Hamid Rajaeian

, Saeid Hosseinzadeh

, Mahboobeh Ashrafi

Abstract: (3390 Views)

Conic snails are slow-moving carnivorous that use a very powerful venom to hunt. This venom is a combination of different toxins from the family of conototoxins. This research was carried out to identify a marine Cone snail, Conus pennaceus quasimagnificus in Shibderaz Cost, South of Qeshm Island. After sampling the venom was isolated using a buffer and centrifuge. Then the lethal dose (LD₅₀) on mice was analyzed and various conotoxins were identified. For the first time, the presence of a conotoxin called “alpha-conotoxin-like” of the A-superfamily was found in the venom of the collected specimens. This peptide was tested for its anti-bacterial activity after being synthesized. Analysis of the isolated DNA showed that the species studied was C. pennaceus and phylogenetical studies showed the closest distance to the C. pennaceus quasimagnificus subspecies. The venom of this cone snake showed the LD₅₀ of 500 mg kg^-1. This peptide showed no effect on different strains of bacteria, however, given the strong inhibitory effect of venom on nicotine acetylcholine receptors (nAChR), it can be further studied for its pain relief effect.

Keywords: Cone Snail, Conus pennaceus quasimagnificus, Conotoxin, Mass-spectrometry, Phylogeny, Persian Gulf

Full-Text [PDF 750 kb] (1115 Downloads)

Type of Study: Research | Subject: Special
Published: 2018/10/15