Specific growth rate and mitotic index in dinoflagellate Symbiodinium sp. isolateed from sea anemone Stichodactyla haddoni

Tahmasebi, Hamideh; Zarei Darki, Behrouz

Volume 7, Issue 2 (Autumn 2017) J. Aqua. Eco 2017, 7(2): 106-114 | Back to browse issues page

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Tahmasebi H, Zarei Darki B. Specific growth rate and mitotic index in dinoflagellate Symbiodinium sp. isolateed from sea anemone Stichodactyla haddoni . J. Aqua. Eco 2017; 7 (2) :106-114
URL: http://jae.hormozgan.ac.ir/article-1-739-en.html

Specific growth rate and mitotic index in dinoflagellate Symbiodinium sp. isolateed from sea anemone Stichodactyla haddoni

Hamideh Tahmasebi

, Behrouz Zarei Darki ^*

Abstract: (4236 Views)

The cultivation techniques of dinoflagellates is often problematic due to their sensitivity to hydrodynamic (shear) stress. For this study, sea anemone was collected from the east coast of Hormuz Island. First, we extracted symbiotic dinoflagellate, Symbiodinium sp. from Stichodactylla haddoni using manually homogenization. After transferring to the laboratory, samples were cultured in different mediums of ASP₁₂, ASP₈, F₂-Gillard and L₁, under temperatures of 17±1, 23±1 and 29±1 °C. The amount of light was about 100 µ mol.photon.m^-2.s^-1. Then, microalgae were cultured again in the selective medium ASP12. Cell counting was performed using a Neubauer chamber every day. Growth and mitotic index were measured during the period of cultivation. Maximum mitotic index, cell numbers and growth rates were observed at 23±1 °C at a rate of 40%, 12×10⁵ cell/ml and 0.086 d^-1,respectively. The results showed significant differences between cell numbers and growth rates of Zooxanthellae at three different temperatures (P <0.05). In conclusion, this study showed that environmental factors such as temperature can affect the growth rate of dinoflagellate, Symbiodinium sp.

Keywords: Dinoflagellate, Dinophyta, Marine microalgae, Biotechnology, Persian Gulf, Symbiodinium

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Type of Study: Research | Subject: Special
Published: 2018/05/7