To investigate the relationship between the length and weight of Neogobius melanostomus, 5145 fish were captured using nets from March to February 2018. The maximum total length and weight for females were recorded at 12.2 cm and 28.08 grams, while for males, the maximum total length and weight were 11.3 cm and 33.17 grams, respectively. The length-weight relationship for both female and male populations of N. melanostomus in Gorgan Bay across the four seasons (spring, summer, autumn, and winter) showed a high coefficient of determination. The regression slope values (b) for this species in different seasons were significantly different from the isometric growth coefficient of 3 (p < 0.05). Specifically, the t-values were 3.73 for the winter population, 10.80 for the autumn population, 21.25 for the summer population, and 21.71 for the spring population, indicating positive allometric growth throughout the year. The Pauli test confirmed this growth pattern in the N. melanostomus population of Gorgan Bay. Seasonal condition coefficients showed that females had the highest values in summer and winter, while males displayed the highest values in winter.