Volume 8, Issue 1 (Summer 2018)                   J. Aqua. Eco 2018, 8(1): 30-40 | Back to browse issues page

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Hassanzadeh N, Malvandi H. Investigation of Toxicological Effects of di-2-ethylhexyl with Fish Embryo Test (FET) in Zebrafish (Danio rerio). J. Aqua. Eco 2018; 8 (1) :30-40
URL: http://jae.hormozgan.ac.ir/article-1-659-en.html
Abstract:   (4907 Views)
Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer in a variety of consumer products. Given the adverse toxicity effects of DEHP on living organisms, its high consumption and dispersal has been caused a serious concern. . Nowedays, the Fish Embryo Test (FET) method is widely used among the conventional methods of toxicology .The aim of the this study was to determine the acute toxicity of Di(2-ethylhexyl)phthalate on zebrafish (Danio rerio) embryo using FET method to assess both lethal and sublethal endpoints and anomalies effects over 5 days . Zebrafish embryos (3 hours post-fertilization) were exposed to different concentrations of DEHP (0.05, 5, 20, 35, 50 and 100 µg/l) for 120 h, then, lethal and sub-letahl endpoints of embryos evaluated and data were analized using Probit software. The LC50, NOEC and LOEC values obtained for DEHP were 46.14, 12.08 and 22.06 µg/l, respectively. Also, There were abnormalities including  coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac, malformation of tail and lack of heartbeat in the embryos. This study showed that DEHP is a teratogenic pollutants and the application of FET method is / an alternative method for developmental toxicity tests to predict effects of emerging pollutants on aquatic organisms.
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Type of Study: Research | Subject: Special
Published: 2018/07/15

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