Inhibitory effect of acetylcholinesterase and caspase activity of human breast cancer cells (MCF-7) treated with filtered extracts of the sea anemone oral disk (Stichodactyla haddoni)

Yavari, Leila; Heidari, Behrooz; Khodabandeh, Saber; Ghafori, Hosein; Hadavi, Mahvash

Volume 8, Issue 1 (Summer 2018) J. Aqua. Eco 2018, 8(1): 92-103 | Back to browse issues page

‎ 20.1001.1.23222751.1397.8.1.9.1

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Yavari L, Heidari B, Khodabandeh S, Ghafori H, Hadavi M. Inhibitory effect of acetylcholinesterase and caspase activity of human breast cancer cells (MCF-7) treated with filtered extracts of the sea anemone oral disk (Stichodactyla haddoni). J. Aqua. Eco 2018; 8 (1) :92-103
URL: http://jae.hormozgan.ac.ir/article-1-698-en.html

Inhibitory effect of acetylcholinesterase and caspase activity of human breast cancer cells (MCF-7) treated with filtered extracts of the sea anemone oral disk (Stichodactyla haddoni)

Leila Yavari

, Behrooz Heidari ^*

, Saber Khodabandeh

, Hosein Ghafori

, Mahvash Hadavi

Abstract: (3195 Views)

The present study investigated the inhibitory effects of the oral disk extract of sea anemone, Stichodactlya haddoni on acetylcholinesterase and caspase activity. The sea anemone samples were obtained from the intertidal zone of the Hormuz Island in the Persian Gulf. Millipore ultrafiltration was carried out to separate different fractions of the crude extract. Ellman method was used to measure acetylcholinesterase inhibition. The activity of caspase 3 was measured using a calorimetric assay kit of Caspase in breast cancer cell MCF-7. At the end of the experiment, the highest percentage of inhibition of the acetylcholinesterase was observed in 6 mg/ml of the 30-100 kDa fraction (p<0.05). The activity of caspase 3 in MCF-7 treated with concentrations of 400, 600 and 800 μg/ml from the fraction 100-30 kDa were significantly different after 12, 24 and 48 hours (p<0.05). The concentration of 600 μg/ml in the desired fraction showed the highest caspase activity after all the three mentioned times. In conclusion, the protein toxins of the sea anemone, S. haddoni, with a molecular weight of 30 to 100 kD can have anti-Alzheimer's effects by inhibiting acetylcholinesterase enzyme and the lethality effects on the breast cancer cell by activating caspase 3 and possibly inducing apoptosis.

Keywords: Sea anemone, acetylcholinesterase, caspase, protein.

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Type of Study: Research | Subject: Special
Published: 2018/07/15