Nowadays, the importance of using natural materials and replace them with high-risk chemicals in aquaculture to produce healthy food is completely obvious. This study was carried out to determine the lethal concentration (LC50 96h) of Sodaby plant (
Haplophyllum virgatum) extract in
Aphanius dispar. This experiment was carried out with five treatments and three replicates in static condition according to O.E.C.D method within 96h. After adaptation, the 150 fish with average weight 0.61±0.18 g were exposed to different concentrations (0, 50, 100, 200 and 300) of Sodaby plant extract and behavioral changes were recorded. Minitab software and Probit analysis were used to determine the lethal concentration (LC
50). The results showed that the LC
50 value of Sodaby plant extract in 96 hours was 57.996 mg.L
-1 in
A. dispar. The Maximum acceptable toxicant concentration (MATC) and the lowest observed effect concentration (LOEC) value of this plant extract were determined 5.7996 and 25.269 mg.L
-1, respectively.