Journal of Aquatic Ecology

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The effect of stocking density and dietary aflatoxin B1 along with rosemary and thyme powders on digestive enzymes activity was evaluated in rainbow trout. A total number of 600 fish with an average weight of 90±3 were randomly allotted among six experimental groups consisting of two stocking densities (15 and 45 kg.m^-3), aflatoxin contaminations (0 and 50 ppb) along with the inclusion of g 4% rosemary and thyme powder. Each treatment was replicated thrice and the experiment lasted for 45 days. Stocking at high density and receiving aflatoxin B1 contaminated diet resulted in higher digestive enzymes activity including amylase, lipase and alkaline protease in comparison with group stocked at lower density and fed aflatoxin free diet (p<0,05). It seemed that the inclusion of an herbal blend, consisting of rosemary and thyme powder could not mitigate pathologically elevated digestive enzymes activity caused by dietary aflatoxin contamination (p>0.05). Further future research is required to precisely elucidate the underlying mechanisms and importance of such culminated digestive enzymes activity due to stocking density and dietary aflatoxin contamination on the fish digestive physiology.
 

Keywords: aflatoxin, rosemary, thyme, rainbow trout

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